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To produce γ-linolenic acid by a mold, cultural conditions of Mortierella isabellina IFO 8183 were investigated. It was found that the increase of initial pH resulted in the decrease of the γ-linolenic acid content and the increase of the C1N ratio of medium resulted in the increase of the lipid cement. Addition of sodium acetate into the medium resulted in the increased of cell yield, lipid yield, γ-linolenic acid content and γ-linolenic acid productivity. Under the optimum coditions(glucose, NH_(4)N0_3, C/N ratio of 40, pH 6.0, 30℃ and 0.5% of sodium acetate), the following results were obtained: cell yield, 0.347(g dry biomass/g glucose; lipid yield, 0.18(g lipid/g glucose); lipid content, 0.52(g lipid/g dry biomass); γ-linolenic acid content, 60(mg γ-linolenic acid/g lipid); maximum γ-linolenic acid concentration, 347 mg/1 after incubation of 8 days.
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